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. 2020 Feb 21;9(5):e014397. doi: 10.1161/JAHA.119.014397

Figure 3.

Figure 3

Neutrophil number increases following myocardial ischemia reperfusion injury. A, Neutrophil number in tail vein blood of sham group reached climax at 12 hours, while number of neutrophils after myocardial ischemia reperfusion injury rose progressively until 24 hours and it remained high at 48 hours. (n=4). B, Fluorescence‐activated cell sorting analysis of hearts showed that neutrophil number in cardiac tissue became maximum 24 hours after myocardial ischemia reperfusion injury and remained high at 48 hours, which was significantly different from that of sham group. (n=6). C and D, Hematoxylin and eosin and immunofluorescence staining indicated that neutrophils had infiltrated into heart 24 hours after ischemia reperfusion, compared with sham group. (n=4). HE indicates hematoxylin and eosin; MI/R indicates myocardial ischemia reperfusion. (Data are presented as mean±SD, ***P<0.001 vs sham, unpaired Student t‐test).