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. 2020 Feb 21;9(5):e014397. doi: 10.1161/JAHA.119.014397

Figure 6.

Figure 6

Immune cell numbers changed after MSCs infusion at the peak of inflammation. A, Gating strategy of fluorescence‐activated cell sorting analysis. Neutrophils were recognized by CD45+Gr+, Macrophages were CD45+Gr‐CD68+, and M2 macrophages were CD45+Gr‐CD68+CD163+. B, When mesenchymal stem cells were given at the peak of inflammation (24 hours after reperfusion), White blood cell absolute numbers and percentage of white blood cells in cells collected from hearts were decreased at 48 and 72 hours after reperfusion, neutrophils’ absolute numbers and percentage of neutrophil in white blood cells were significantly decreased at 48 and 72 hours after reperfusion, macrophages’ absolute numbers were decreased at 72 hours but the percentage of macrophages in white blood cells were significantly increased, M2 macrophages’ absolute numbers were unchanged while percentage of M2 macrophages in all macrophages were significantly increased. (n=6). M2 indicates M2 macrophages; MI/R, myocardial ischemia reperfusion; MSC, mesenchymal stem cells; WBC, white blood cells; SSC indicates side scatter. (Data are presented as mean±SD, ### P<0.001 vs MI/R, unpaired Student t‐test).