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. Author manuscript; available in PMC: 2020 Jul 5.
Published in final edited form as: Neuron. 2019 May 29;103(2):242–249.e4. doi: 10.1016/j.neuron.2019.04.039

Figure 4. Activity of the Reward Circuits Is Separable from Thirst Satiation Signals.

Figure 4.

(A) A diagram of optical recording of DA release by dLight1.3. A representative image of dLight expression is shown.

(B) DA release is induced by appetitive (Ensure) stimulus and suppressed by aversive (footshock) stimulus (n = 7 mice).

(C) dLight fluorescence changes are shown during oral ad lib intake and IG infusion (n = 7 mice). Spontaneous drinking induced robust DA release in the NAc compared to empty control regardless of liquid type (left). For empty control experiments, DA release was observed transiently prior to lick due to reward expectation. By contrast, IG infusion of fluid had no effect on DA release (right, n = 7 mice).

(D) Quantified data of dLight responses during 4 s around the first lick (left) or 60 s (right) after the first lick or IG infusion (n = 7 mice).

(E) A schematic for activating thirst satiation circuits in the LT by hM3Dq while measuring DA release in the NAc by dLight1.3.

(F) Chemogenetic stimulation of SFOGLP1r and MnPOGLP1r neurons attenuates water intake under dehydrated conditions (n = 6 mice).

(G) By contrast, the same stimulation paradigm did not induce DA release (n = 6 mice).

(H) A diagram of operant task. Mice were initially trained to associate lever press and water reward. After extinction sessions (see Figure S4F), animals were subjected to reinstatement paradigms with either IG or oral water reward (left). In IG sessions, animals received water through a gastric catheter on an FR3 schedule (middle). In oral sessions, the same amount of water reward was provided through a spout (right, n = 6 mice). Only oral water intake efficiently reinforced lever press behavior.

*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 by two-tailed paired t test; one-way repeated-measures ANOVA (Dunnett’s multiple comparisons) or two-way repeated-measures ANOVA (Bonferroni’s multiple comparisons). Data are presented as mean ± SEM. Scale bar, 50 μm. See also Figure S4.

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