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. 2020 Jun 30;11(26):2571–2585. doi: 10.18632/oncotarget.27654

Figure 1. Peripheral blood NK cells of RCC patients have decreased cytotoxic function.

Figure 1

NK cells were isolated from peripheral blood of 5 healthy, cancer-free donors (HD) or 6 patients diagnosed with RCC by negative selection and evaluated for expression of surface markers by multi-color flow cytometry or ability to lyse K562 human erythroleukemia cells. (A) Percentage of CD56+CD16+ pNK cells isolated from HD or RCC patients with mean ± SEM reported. Each symbol represents an independent person. (B) Relative cytotoxic activity of freshly isolated pNK cells from HD or RCC patients for the indicated target:effector ratios. Data are analyzed relative to K562 cells treated with digitonin serving as a positive control for cell death (set = 100% lysis) and plotted as mean ± SEM (n = 5 healthy donors and RCC patients). (C) Concentrations of activated TGFβ in plasma from healthy donors and RCC patients determined by ELISA with mean ± SEM reported. Each symbol represents an independent person. n.s., not significant; * P ≤ 0.05; ** P ≤ 0.01; determined by Student’s t-test.