Figure 1.

Inter- and Intrapatient Variation of Primary Fibroblasts Isolated from Malignant Glioma (MG) Patient Biopsies

(A) Schematic depiction of the cell-engineering process. (B) White light photomicrographs of PF and NHF1 revealed morphology differences between samples. RNA was isolated from biological replicates (n = 3) to assess intra- and interpatient variability. (C) Principal component analysis (PCA) and a Pearson correlation heatmap, based on the entire gene-expression profile of all samples, suggested that interpatient variation exceeded intrapatient variation. Numbers represent biologic replicates. (D) Heatmap revealing the most highly differentially expressed genes between model fibroblast line NHF1 and each PF line. Samples are ordered according to unsupervised hierarchical clustering (p < 0.05, F < 0.0001).