Figure 8.

Identifying Candidate QA Markers

(A) Flowchart describing the approach used to select candidate markers. DEGs with fold-change >2 and GO annotations related to proliferation and migration were compared among all patient cells and selected as candidate markers based on the similarity of their expression profile to functional phenotype. (B and C) Heatmap (B) and GO (C) analysis of most highly differentially expressed genes induced in fast-expanding versus slow-expanding PF revealed enrichment in genes associated with cell proliferation, cell adhesion, and extracellular matrix (ECM) organization. (D and E) Heatmap (D) and GO (E) analysis of most highly differentially expressed genes induced in fast-migrating and slow-migrating iNSC-Ts revealed differences in locomotion, biological adhesion, and regulation of cell migration. (F) Heatmap of the 14 most highly expressed candidate marker genes identified for cell growth, with 3 genes selected based on known function in fibroblasts proliferation or senescence. (G) Heatmap of 30 most highly expressed candidate marker genes identified for tumor tropism, with 3 genes selected based on known function in neural cell motility (n = 3, p < 0.05). Boxes indicate candidate genes selected. (H) Table indicating marker selection.