Figure 4.

Targeted Integration of a Partial cDNA at CFTR Intron 8 Does Not Disrupt the Open Chromatin Profile of the ΔF508/ΔF508 CFTR Locus

Integrative Genomics Viewer (IGV) browser graphics of CFTR TAD regions showing Omni-ATAC profiles of non-CF, ΔF508/ΔF508, and TI-8 ΔF508/ΔF508 ALI cells. The number of sequencing reads is shown in Table S7. Key cis-regulatory elements are marked above the ATAC profiles by arrows. DHS denotes all DNase I-hypersensitive sites across the locus. Below is a magnified panel of the intron 8 region showing appearance of a peak of open chromatin at the TI-8 cDNA insertion site.