Figure 2.

The Effects of Reporter Dosage and the Transfection Time on the BEON Assay

(A) Representative fluorescence and bright-field (merged) images of HEK293 cells transfected with different amounts of the S1-reporter (0.125, 0.25, 0.5, 0.75, 1.0 μg) and 1:1 of ABEmax and S1-gRNA with a total of 2 μg DNA per well in a six-well plate. The images were taken at day 3 after transfection. Scale bar: 100 μm. (B) Representative fluorescence and bright-field (merged) images of HEK293 cells transfected with S1-reporter (0.5 μg), ABEmax (0.75 μg), and S1-gRNA (0.75 μg), imaged at different time points after transfection. (C) FACS quantification of GFP⁺ cells for conditions described in (A). (D) FACS quantification of GFP⁺ cells for conditions described in (B). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.