Figure 3.

Screening for Optimal Base Editors Using the BEON Assay

(A) Representative bright-field (upper) and GFP fluorescence (lower) images of HEK293 cells without transfection (control), transfected with the reporter alone, or co-transfected with S1-gRNA, S1-reporter, and one of the base editors: ABE7.10, xABE7.10, or ABEmax. Scale bar: 100 μm. (B) Representative flow cytometry plots of HEK293 cells as treated in (A). (C) Quantification of GFP⁺ population by FACS analysis for the HEK293 cells as treated in (A). ∗∗p < 0.01; ∗∗∗∗p < 0.0001.