Figure 2.

Kinetics of CAR Downregulation and Its Reversal

GD2-LID T cells were incubated with 1 μM shield-1 or vehicle, and CAR expression was evaluated at serial time points by flow cytometry. At 24 h after shield-1 addition, T cells were washed several times to remove shield-1 or vehicle, returned to culture, and stained for CAR at 24 and 48 h after wash-out. Percent maximum CAR expression is plotted as for Figure 1C. The connecting line is the average of three to four different T cell donors, and each data point represents a different donor, with the same symbol representing a particular donor across time points.