Figure 5.

PASTA domain Ser/Thr protein kinases function independently of the Rpfs to affect spore germination and growth. A, WT, rpf null (5×Δrpf), and PASTA Ser/Thr kinase null (3×ΔPASTA) spores were monitored for germination using light microscopy over the course of 8 h. Data are representative of three independent replicates (n ≥ 200 spores per strain per time point). B, growth profiles of the WT, rpf null, and Ser/Thr PASTA kinase null strains in new minimal medium with phosphate liquid medium. Data presented are the average of three independent replicates ± S.E.