Table 2.
LC-Q-TOF analysis of select muropeptides released from insoluble M. luteus peptidoglycan by RpfA-E
| Fraction No.1 | Annotation2 | Expected | Observed (m/z) |
z | ||||
|---|---|---|---|---|---|---|---|---|
| RpfA | RpfB | RpfC | RpfD | RpfE | ||||
| Rpf-soluble reaction products | ||||||||
| 1 | G-anhM(Penta) | 468.2050 | 468.2131 | 468.2112 | 468.2103 | NA3 | 468.2121 | 2 |
| 2 | G-anhM(Penta-Ala) | 503.7250 | 503.7322 | 503.7326 | 503.7330 | NA | 503.7309 | 2 |
| Rpf-insoluble reaction products | ||||||||
| 3 | G-anhM(Penta) | 468.2050 | 468.2118 | 468.2117 | 468.2120 | 468.2137 | 468.2123 | 2 |
| 4 | G-anhM(Penta-Ala) | 503.7250 | 503.7300 | 503.7309 | 503.7300 | 503.7320 | 503.7306 | 2 |
| 5 | G-M*-G-M(Penta)-G-anhM | 616.6200 | 616.9644 | 616.6293 | 616.9655 | 616.6328 | 616.6308 | 3 |
| 6 | G-M-G-M(Penta)-G-anhM(Penta) | 782.0300 | 782.O290 | 782.O266 | 782.O266 | 782.O297 | 782.O269 | 3 |
| 7 | G-M-G-M-G-anhM(Penta-Ala) | 979.9350 | 979.9418 | 979.9393 | 979.9386 | 979.9434 | 979.9394 | 2 |
1The muropeptide fractions correspond to those of the RP-HPLC separation presented in Fig. 2, A and B.
2Identification of each muropeptide was made by tandem Q-TOF-MS analysis of each parent ion (data not shown); G, GlcNAc; M, MurNAc; anhM, 1,6-anhydroMurNAc; penta, l-Ala-d-Glu-(Gly)-l-Lys-d-Ala; *, O-acetylation.
3NA, not observed.