Table 1.
Strains employed in this study
| Description | Name | Parental strain | Genotype | Gene targeted | Selection marker | Reference |
|---|---|---|---|---|---|---|
| RH (type 1) | WT | 21 | ||||
| Δgat1/RH | MM12, cl.A8 | RH | Δgat1 | gat1 | DHFR | This report |
| gat1+/Δgat1/RH | MM21, cl.E12 | MM21 | Δuprt/gat1::gat1-Ty | uprt | Δuprt | This report |
| RHΔΔ | RH | Δku80/Δhxgprt | 22 | |||
| Δgat1-1/RHΔΔ | KR10, cl.A1 | RHΔΔ | Δku80/Δgat1 | gat1 | HXGPRT | This report |
| Δgat1-2/RHΔΔ | MM16, cl.B7 | RHΔΔ | Δku80/Δhxgprt/Δgat1 | gat1 | DHFR | This report |
| gat1+/Δgat1-2/ RHΔΔ | MM24, cl.G10 | MM16 | Δku80/Δhxgprt/Δuprt/tub::gat1-3×HA | uprt | Δuprt | This report |
| Me49-RFP (type 2) | MM8, cl.A10a | Me49 | rfp+ | 23 | ||
| Δgat1/Me49-RFP | MM14, cl.B5 | Me49-RFP | Δgat1 | gat1 | DHFR | This report |
a High RFP expression level clone from original source.