Figure 5.

Cdc13-DBD does not form a stable complex with either a G-hairpin or a G-quadruplex and does not interfere with their folding kinetics. A, imino regions of the 1D ¹H NMR spectra of an equimolar mixture of ONG1 (top), ONG9 (middle), ONG11 (bottom), and Cdc13-DBD were acquired as a function of time (indicated). B, time course of the folding process for ONG1 (black box), ONG9 (blue box), and ONG11 (red box) in the presence of equimolar amounts of Cdc13-DBD as estimated from normalized time-dependent changes in the intensity of imino signals (I) from NMR spectra presented in A. The imino signal intensities at t = 30 and t = 90 min were normalized with respect to those acquired at t = 150 min. The NMR spectra were acquired using a 1-1 echo pulse sequence with an excitation maximum set to 12 ppm. C, overlay of the imino regions of the 1D ¹H NMR spectra of ONG1 (top), ONG9 (middle), and ONG11 (bottom) measured in K⁺TD buffer in the absence (black) and in the presence of equimolar amounts of Cdc13-DBD (red).