Figure 1.

Unstructured and structured regions of TOMM34 contain PKA-targeted serine residues. a, prediction of TOMM34 disordered regions (43). The red dashed line denotes the prediction threshold. TOMM34 domain structure with the indicated location and sequence context of the preferentially PKA-targeted serine residues is depicted. The regions of TOMM34 implicated in the interaction with HSP70 (TPR1 domain and interdomain linker) are marked. b, summed extracted ion chromatograms for selected TOMM34 peptides covering PKA phosphorylation sites Ser⁹³ (peptides 79–95), Ser¹⁶⁰ (peptides 145–170), and Ser²⁸⁰ (peptides 265–281). Traces for nonphosphorylated (blue) and phosphorylated (red) versions of the peptides derived from nonphosphorylated TOMM34 and PKA-phosphorylated TOMM34 (pTOMM34) are shown. The level of the targeted sites phosphorylation is indicated as percentages.