Skip to main content
. 2020 May 19;295(27):9244–9262. doi: 10.1074/jbc.RA120.013077

Figure 6.

Figure 6.

Clstn3 is expressed in glutamatergic, PV-positive, and SST-positive neurons in the adult mouse hippocampus. A-C, expression of Clstn3 mRNA (red) in various hippocampal regions, as visualized by in situ hybridization using RNAscope technology. Nuclei were stained with DAPI (blue). Representative image (A) and quantitative analyses (C) of RNAscope data are shown. Scale bar: 200 μm for larger color image on the left, and 20 μm for images on the right. B, expression of Clstn3 mRNA (red) in CamKIIα-positive glutamatergic, PV-positive, and SST-positive neurons in hippocampal regions, shown by staining for the indicated gene probes (green) by RNAscope-based in situ hybridization, followed by counterstaining with the nuclear dye DAPI (blue). Scale bar: 20 μm. D, expression of Clstn3 protein in the hippocampus of Clstn3flox/flox (Ctrl) or Nestin-Cre;Clstn3flox/flox (Nestin-Clstn3) mice at P42 (6 weeks), as visualized by immunohistochemistry using anti-Clstn3 antibodies (JK091). Nuclei were stained with DAPI (blue). Scale bar: 200 μm for larger images on the left, and 20 μm for images on the right.