Figure 7.

Accumulation of complex sphingolipids in NPC1^KO cells is reduced by SK1-A treatment. NPC1^KO CHO cells were treated without or with SK1-A (10 μm) for 24 h. Lipids were extracted from the indicated CHO cells, and ceramide (A), monohexosylceramide (B), and sphingomyelin species (C) were measured by LC-ESI-MS/MS. Different chain-length species are shown; numbers indicate N-acyl chain length followed by the number of double bonds in the fatty acid. Data are means ± S.D. (error bars) of biological replicates and representative of three independent experiments. *, p < 0.05 compared with WT; #, p < 0.5 compared with NPC1^KO as determined by one-way ANOVA (totals) or two-way ANOVA (species) followed by Tukey's post hoc analysis.