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A, native MS of OmpF-trypsin digested ColN. Blue circles, OmpF; yellow circles, OmpF + 3375 Da ColN peptide. B, immunodiffusion assay of OmpF and colicin N constructs, four 3-mm wells were cut out of a potassium phosphate buffer, 0.5% agarose gel, pH 6.65, 1% β-OG, 20 µl of each protein was added to the wells at a concentration of 17 μm, plates were incubated overnight at room temperature to allow for precipitation.
