Figure 4.
A, OmpF single channel conductance in planar lipid bilayers upon addition of 1 μm ColN2-19 from the extracellular (cis) face of the OmpF channel. (B) OmpF single channel conductance in planar lipid bilayers upon addition of 1 μm ColN2-19 from the periplasmic (trans) face of the OmpF channel. Conductance measurements were performed in 100 mm potassium chloride, 20 mm potassium phosphate, pH 6.5, at room temperature. Arrows indicate the different levels of membrane conductance and associated numbers represent the number of open channels associated with conductance level.