Figure 3.

ubiU_Pa, ubiV_Pa, and ubiT_Pa are essential genes for anaerobic UQ₉ biosynthesis and for denitrification. Shown are representative growth curves of WT PAO1 and ubiTUV-KO strains grown in denitrification medium (A) or aerobically in LB medium (C). B, Quantification of cellular UQ₉ content (n = 3) in lipid extracts from WT PAO1 and KO cells grown aerobically in LB medium (white bars) (P > 0.05 by unpaired Student's t test) or in denitrification medium (gray bars) (****, P < 0.0001 by unpaired Student's t test). Error bars represent S.D.