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Spectral characterization of UbiVPa and its variants
| Protein | A280/A410 | Contenta (nmol/nmol protein) |
|
|---|---|---|---|
| Iron | Sulfur | ||
| UbiVPa WT | 5.8 | 3.90 ± 0.03 | 3.40 ± 0.20 |
| UbiVPa C39AC180A | 9.0 | 1.10 ± 0.16 | 1.60 ± 0.19 |
| UbiVPa C193AC197A | 7.8 | 2.90 ± 0.05 | 3.00 ± 0.12 |
| UbiVPa C39AC193AC197A | ND | ND | ND |
a Iron and sulfur quantification of UbiVPa and its variants. Shown are the metal content and UV-visible properties after anaerobic reconstitution of their [Fe-S] clusters for WT and variants. ND, not determined.
