Figure 7.

A, plot showing relation between the numbers of effective reads sequenced (i.e. without rRNA/tRNA) and the number of annotated genes predicted to be potentially translated based on REPARATION (≥3 reads, orange) or REPARATION prediction plus an RCV threshold (≥0.355, blue). Results for data with both estimations (i.e. RNA-Seq available) are connected via dashed lines. B, relation between numbers of annotated genes predicted to be translated for subsampled data sets having a high sequencing depth originally (samples from Woo_15; each in triplicate; SRR1734437; SRR1734439; SRR1734441). Gene number estimations are based on REPARATION predictions as before.