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. 2020 May 12;295(27):9134–9146. doi: 10.1074/jbc.RA120.013040

Figure 1.

Figure 1.

Modular structure of Jd1381 and its truncated versions. A, the full-length enzyme Jd1381 contains an N-terminal signal peptide (residues 1–31; black line), an AA10 type LPMO (LPMO10, residues 32–173; purple), a CBM5 domain (residues 200–251; green), and a C-terminal GH18 type chitinase (Chi18, residues 280–651; light blue). Two linkers (black lines) connect the CBM5 domain to the LPMO10 and Chi18 domains, containing 26 and 28 residues, respectively. The four additional pictures show the domain organizations of truncated versions of Jd1381 produced in this study, named JdLPMO10, JdLPMO10-CBM5, JdCBM5-Chi18, and JdChi18. B, SDS-PAGE gel showing the recombinant enzymes produced and purified in this study. The left lane shows protein size markers with their sizes indicated in kDa.