Figure 6.

The impact of Jd1381 on α-chitin particles. A, SEM image showing an α-chitin particle before enzymatic treatment. B and C, SEM images showing α-chitin particles after treatment with Jd1381 in the presence (B) and absence (C) of AscA. Note that the particles shown in B were exposed to an active LPMO domain, whereas the particles shown in C were not. The reaction mixtures contained 1 μm Cu(II) saturated enzyme, 10 g/liter chitin, and, when indicated, 1 mm AscA, in 20 mm BisTris, pH 6.0, and were incubated at 40 °C with shaking at 1000 rpm for 3.5 h prior to imaging. The control reaction (A) did include AscA but lacked the enzyme. The scale bars indicate 1 μm in A and C and 2 μm in B.