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. 2020 Jul 1;12:5243–5254. doi: 10.2147/CMAR.S237323

Figure 5.

Figure 5

MiR-101 targeted Mcl-1 3ʹUTR and inversely regulated the expression of Mcl-1. (A) Predicted binding sequences between miR-101 and Mcl-1 3ʹUTR. The nucleotides highlighted in green colour were indicated as mutated sites. (B, C) Panc-1 cells were co-transfected with miRNAs (mimics NC or miR mimics) and reporter vectors (Mcl-1 3ʹUTR-WT or Mcl-1 3ʹUTR-MUT), and 48 h later, relative luciferase activity was evaluated by Dual-Luciferase Reporter Assay System. (D, E) PANC-1 cells were transfected with mimics NC or miR mimics, and 24 h later, the mRNA and protein expression of Mcl-1 was determined by qRT-PCR and Western blot assay, respectively. (F, G) PANC-1 and SW1990 cells that were treated with Honokiol (50 µM) or vehicle were transfected with miR inhibitors or inhibitors NC, and 24 h later, the mRNA and protein expression of Mcl-1 was determined by qRT-PCR and Western blot assay, respectively. N = 3. *P<0.05, **P<0.01 and ***P<0.001.