Figure 6.

Mcl-1 overexpression counteracted tumor-suppressive effects of Honokiol on pancreatic cancer cells. (A, B) PANC-1 cells were transfected with pcDNA3.1 or pcDNA3.1-Mcl-1, and 24 h later, the mRNA and protein expression of Mcl-1 was determined by qRT-PCR and Western blot, respectively. (C–F) PANC-1 and SW1990 cells that were treated with Honokiol (50 µM) or vehicle were transfected with pcDNA3.1 or pcDNA3.1-Mcl-1, and 24 h later, cell viability was determined by MTT assay (C), cell apoptosis was evaluated with Caspase-3 Activity Assay Kit (D) and flow cytometry (E), and cell invasive ability was assessed with Transwell invasion assay (F). N = 3. **P<0.01 and ***P<0.001.