Table 1.
Protein | PDB | ΔΔG Range (kcal/mol) | # Mutations | Monopeptide MUE (kcal/mol) | Tripeptide MUE (kcal/mol) |
---|---|---|---|---|---|
S. nuclease | 1EY0 | −1.0 to 2.1 | 27 | 2.01 | 0.78 (1.14) |
Barnase | 1BNI | 0.5 to 4.5 | 13 | 0.93 | 1.11 |
T4 lysozyme | 1L63 | −0.6 to 2.7 | 16 | 1.13 | 0.64 |
T4 lysozyme | 2LZM | −0.8 to 2.3 | 13 | 2.51 | 0.91 (1.19) |
Protein G | 1PGA | −0.8 to 3.5 | 10 | NA | 1.11 |
Ribonuclease | 1RGG | −0.8 to 1.3 | 8 | NA | 0.58 |
The ΔΔG refers to the experimental free energy difference between the wild-type and the mutants. A negative value indicates stabilization and positive value destabilization. The numbers in parenthesis are before outlier analysis.