Figure 4.

(A) Time lapse fluorescence images of a fusing vesicle (magenta) with transfer of its content across the PSM. Scale bar, 2 μm. (B) Fluorescence intensity time traces obtained from ROI 1 and 2 shown in (A). After docking (I), the vesicle fuses and releases its content (II) into the aqueous compartment underneath the adjacent f-PSM, where it leads to an increase in fluorescence intensity visible in ROI 2. (C) Schematic illustration of a vesicle docked at the edge of a pore and releasing its content into the underlying aqueous compartment. The figure is not drawn to scale. To see this figure in color, go online.