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. 2020 Apr 20;5(7):1071–1075. doi: 10.1016/j.ekir.2020.04.010

Figure 1.

Figure 1

Sera from minimal change disease (MCD) patients increase human umbilical vein endothelial cell (HUVEC) permeability evaluated by impedance in vitro. (a) The xCELLigence Real-Time Cell Analyzer system (ACEA Biosciences, Inc., San Diego, CA) allows the evaluation of the permeability of a monolayer of endothelial cells (i.e., HUVECs), seeded in the wells of an electronic microtiter plate. The cellular impedance is measured from the beginning of HUVEC culture, and HUVECs are stimulated with sera once they have reached confluence. (b) Results obtained after stimulation of confluent HUVECs by MCD patient sera (n = 10) and healthy volunteer (HV) sera (n = 11). The steeper slope observed with MCD compared to HV sera reflects an increased permeability of HUVEC monolayer. The slopes of impedance curves were measured between T0 and T45 minutes from the beginning of stimulation with sera. The MCD patient sera induce a greater permeability than the HV sera. ∗P = 0.0276.