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. 2020 Apr 20;5(7):1071–1075. doi: 10.1016/j.ekir.2020.04.010

Figure 2.

Figure 2

Sera from minimal change disease (MCD) patients increase human umbilical vein endothelial cell (HUVEC) permeability to sodium in vitro. (a) The passage of sodium fluorescein (NaF, 276 Da) is measured across a semipermeable membrane from the Transwell system at different times (15, 30, and 45 minutes and 1 hour) after stimulation of confluent HUVECs by patient sera. (b) Results are expressed as the ratio of the fluorescence intensity between the lower and upper chambers of the Transwell system (Thermo Fisher Scientific, Waltham, MA). More passage of NaF was observed at T15, T45, and T60 minutes after stimulation with MCD patient sera (n = 5) compared to healthy volunteer (HV) sera (n = 5). ∗P < 0.05.