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. 2020 Apr 20;5(7):1071–1075. doi: 10.1016/j.ekir.2020.04.010

Figure 3.

Figure 3

The increased permeability of human umbilical vein endothelial cells (HUVECs) to sodium is reversed by amiloride and by aprotinin. (a) Measurement of the sodium fluorescein (NaF) passage through a semipermeable membrane of the Transwell system (Thermo Fisher Scientific, Waltham, MA) after stimulation of HUVECs by sera from minimal change disease (MCD) patients or healthy volunteers (HVs), with or without addition of amiloride (1 μM), 15 minutes after the start of stimulation. The results are expressed as the ratio of the fluorescence intensity between the lower and upper chambers. The increased permeability of HUVECs to NaF induced by MCD patient sera is reversed by amiloride (P = 0.0499). Amiloride has no effect on HUVEC permeability after stimulation with HV sera. ∗P < 0.05. (b) Measurement of the sodium fluorescein (NaF) passage through a semipermeable membrane of the Transwell system after stimulation of HUVEC by sera from MCD patients or HV, with or without adjunction of aprotinin (100 μg/ml), 15 minutes after the start of stimulation. The increased permeability of HUVEC to NaF induced by MCD patient sera is reversed by aprotinin (P = 0.0317). Aprotinin also reduced the permeability of HUVECs after stimulation with HV sera. ∗P < 0.05.