|
Microorganism
|
Method of purification
|
Yield %
|
| Thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. Isolate ANT-6 |
Partial purification by ethanol precipitation |
- |
| Marine Vibrio sp. |
Substitute affinity method by insoluble corn stratch |
78 |
|
Bacillus subtilis
|
High-speed counter-current chromatography |
73 |
|
Bacillus amyloliquefaciens
|
Primary precipitation by ammonium sulphate then final purification by ion exchange chromatography |
- |
| Thermostable α -amylase from Bacillus sp. PS-7 |
Partial purification with ammonium sulphate and further purification by Sephadex G-75 gelfiltration followed by phenyl agarose 4XL hydrophobic interaction chromatography. |
- |
|
Bacillus amyloliquefaciens
|
Affinity precipitation by alginate as the affinity matrix. |
96 |
| - |
Affinity adsorption chromatography |
95 |
| - |
Expanded bed chromatography |
69 |
