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. 2020 May 11;10(3):464–471. doi: 10.34172/apb.2020.057

Table 1. Transformation efficiency of genes into Artemisia annua L. leaves using vacuum infiltration method (VIL), using syringe infiltration method (VSL) and hairy root using vacuum infiltration method .

Sample Blue Area to Total Area (%)
VIL VSL HR
WT 0 0 0
AGL1 0 0 0
Ads 58.47±1.27 39.04±9.01 80.21±6.38
ads-p19 97.13±1.2 92.55±1.39 96.00±0.26
p19 98.25±0.54 89.82±1.08 99.42±0.55
Co-T 85.92±0.13 65.22±6.87 91.17±0.79

Note:WT = wild type leaves, Transformed leaves withAgrobacterium tumefaciens, AGL1 = without plasmid and gene, ads = single plasmid with ads gene, ads-p19 = single plasmid with ads and p19 genes, p19 = single plasmid with p19 gene, Co-T = double plasmids, one with ads, another with p19 gene.