Figure 2.

Monocytes transmigrate across a human umbilical vein endothelial cell monolayer in response to IL-17. A sample of 3 × 10⁵ (a–c) CD14⁺⁺CD16⁻ or (d–f) CD14⁺⁺CD16⁺/CD14⁺CD16⁺⁺ monocytes was added to the upper surface of monolayers of human umbilical vein endothelial cells (HUVECs) previously treated with IL-17, IFN-γ, or IL-17/IFN-γ; culture medium alone was used as a negative control. The monocytes were allowed to transmigrate across the HUVEC monolayers in the presence of CCL2, CX3CL1, or culture medium for 3 hours. All data are presented as the migration index, which relates the number of cells that migrated in response to the indicated stimulus to the number of cells that migrated in response to the negative control. White column: STEMI: black column: post-STEMI; n = 11. ^∗p < 0.05.