Fig. 1. MCV ST transactivates components of the LSD1 complex.

a-c. Two biological replicates of MAX (MAX-1 and MAX-2), EP400 (EP400–1 and EP400–2), and MCV ST (ST-1 and ST-2) ChIP-seq reveal that MCV ST in a complex with MAX and EP400 binds to the promoters of LSD1 (KDM1A), RCOR2 and INSM1. The UCSC genomes browser was used to visualize peaks⁴¹. d. RT-qPCR assesses EP400, RCOR2, and LSD1 levels after expression of inducible EP400 or control shRNA in MKL-1. Data are shown as mean of n=3 ± SD; two-sided t-test, *P<0.05. e. ChIP-qPCR indicates that MAX, EP400, and MCV ST bind specifically to RCOR2, LSD1, and ATOH1 promoters in MKL-1. KRT9 promoter and the intergenic region serve as negative controls. Data are shown as mean of n=3 ± SD; two-sided t-test, *P<0.05; **<0.005. f. Immunoprecipitation and mass spectrometry analysis (MudPIT) shows that LSD1 and RCOR2 form a multi-protein complex in MCC cell lines. The Venn diagram shows 123 overlapping binding partners of LSD1 and RCOR2 from four independent mass spectrometry analyses (Source Data 1). g. PHL cells with MCV T antigens (+) have increased levels of RCOR2 and LSD1 mRNA. The INSM1 levels were below detection limits in PHL cells. The signals were normalized to RPLP0. Data are shown as mean of n=3 ± SD; two-sided t-test, *P<0.05; **<0.005. h. PHL cells with MCV T antigens (+) have increased protein levels of RCOR2 and LSD1. The experiment was performed at least three times. See Unprocessed gels Figure 1. i. RCOR2 levels increase, and LSD1 binds to RCOR2 in PHL cells transformed with MCV T antigens (+). Immunoprecipitation of LSD1, followed by western blotting, was performed three times. # non-specific band. See Unprocessed gels Figure 1. j. ChIP-qPCR of MAX, EP400, and MCV ST in PHL cells expressing T antigens indicate that T antigens enhance MAX and EP400 binding to RCOR2 promoter. Data are shown as mean of n=3 ± SD; two-sided t-test, *P<0.05; **<0.005.