Figure 1.

The overall workflow conducted to investigate the heterosis mechanism in pigeonpea. Two hybrids and their parental lines were selected for this analysis. Firstly, parental lines and hybrids were characterized phenotypically in 15 and 30 days after sowing. The 15‐days seedling was utilized to prepare the libraries for DNA methylation, small RNA and RNA sequencing. Several bioinformatics pipelines were utilized to decipher the genome‐wide data to answers the biological questions. As mentioned in the figure, we have performed several different analyses of the data sets to understand the heterosis in pigeonpea.