Figure 2.

Molecular mapping of leaf shape gene (HcLMI1). (a) The 3:1 segregation ratio of lobed‐leaf: round‐leaf in the F₂ population indicated a major gene controlling this trait with lobed‐leaf as dominate and round‐leaf recessive (Zhang et al. 2019). (b) LOD score for leaf shape gene mapping, based on a high‐density SNP genetic map, shown in Table S9. (c) Gene structure and variation in a candidate leaf shape gene HcLMI1. Exons and introns are represented by boxes and lines, respectively. The position of the causal micro‐structure variation is marked. Red and blue boxes represent P₁‐’Zanyin No. 1’ and P₂‐‘Fuhong 952’, respectively. (d). Amino acid comparison of Hc LMI1 between P₁‐’Zanyin No. 1’ and P₂‐‘Fuhong 952’. The red line: Homeobox domain. The blue line: HALZ domain. e. Representative leaves from VIGS experiment showing the reversion to round‐like leaf shape (Lobed‐leaf: TRV‐LMI1) in the HcLMI1 silencing treatment; Albino phenotype of HcCLA1 silenced kenaf plants represents successfully establishment of tobacco rattle virus‐induced genes silencing (TRV‐VIGS); Relative transcript levels of candidate genes in the HcLMI1 silenced and control Lobe‐leave plants (n = 3) confirmed the effective knockdown of LMI1. Asterisks represent statistically significant differences as determined by unpaired t tests at P < 0.05.