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. 2020 Mar 4;18(8):1830–1842. doi: 10.1111/pbi.13345

Figure 2.

Figure 2

Pst_13661 suppresses Bax‐induced cell death in Nicotiana benthamiana. (a) Transiently expressing Pst_13661 24 h prior to infiltrating with Agrobacterium strain carrying Bax in Nicotiana benthamiana. eGFP was a negative control, and Avr1b was a positive control. Images were photographed 7 days after inoculation with Agrobacterium carrying the corresponding vectors. (b) Total proteins from Nicotiana benthamiana were extracted at 72 h after infiltration with Agrobacterium, and proteins Pst_13661:HA (28 kD), Avr1b:HA (15 kD) and eGFP:HA (25 kD) were detected by Western blot with anti‐HA antibody. Protein Bax (22 kD) was detected with anti‐Bax antibody.