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. 2020 Mar 4;18(8):1830–1842. doi: 10.1111/pbi.13345

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© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Pst_13661 forms homo‐polymer. (a) Yeast two‐hybrid analysis indicates that Pst_13661 interacts with itself. The transformants grew on the SD‐LW, SD‐LWH and SD‐LWHA with X‐a‐gal. L, leucine; W, tryptophan; H, histidine; A, adenine. (b)Bimolecular fluorescence complementation (BIFC) confirmed the interaction in planta. The eYFP‐Pst_13661 and the empty vector (the C‐terminal of YFP) were used as negative controls. Bar = 50 μm. (c) Co‐immunoprecipitation assay confirmed these interactions in planta. Anti‐HA and anti‐Flag were used to detect protein expression.