Figure 8.

Pst_13661 suppresses chitin‐induced plant defence in N. benthamiana. (a) Callose deposition induced by 200 µg/mL chitin in tobacco leaves transiently expressing Pst_13661: mCherry, Pst_13661m: mCherry or mCherry alone. Images were obtained 24 h after infiltration with chitin. Bar = 200 μm. (b) Pst_13661 suppresses callose spots in N. benthamiana. The number of callose spots per 1‐mm² was assessed with the ImageJ software. Mean and standard deviations were calculated from three biological replicates. Asterisks mark significant difference based on Student’s t‐test (*, P < 0.05; ns, not significant). (c) The expression levels of PR1, PR2 and WRKY12 in N. benthamiana leaves transiently expressing Pst_13661: mCherry, Pst_13661m: mCherry or mCherry after infiltration with chitin were assayed by qRT‐PCR with Act1 as a reference gene for normalization. Mean and standard deviations were calculated from three biological replicates. Asterisks indicate significant differences based on Student’s t‐test (*, P < 0.05; ns, not significant).