Table 2. Optimal conditions for Cas9-RNP–mediated gene deletion in human myeloid cells.

	Monocyte-derived macrophage (M-CSF)	Monocyte-derived DC (GM-CSF + IL-4)
Program	CM-137	CM-137
Buffer	P3	P3
Cell number range per reaction in 20 µl volume	0.5–5 × 106 fresh or thawed monocytes	0.5–5 × 106 fresh or thawed monocytes
Culture conditions following electroporation	Monocyte-derived macrophage culture medium containing M-CSF	Mo-DC culture medium containing GM-CSF + IL-4