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. 2020 Apr 27;217(7):e20191736. doi: 10.1084/jem.20191736

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© 2020 Tenthorey et al.

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Figure 1. — CRISPR/Cas9 targeting of Nlrc4. (A–C) The sequence of the Nlrc4 locus in WT C57BL6/J compared with CRISPR/Cas9-generated (A) Nlrc4^−/−, (B) Nlrc4^S533A/S533A, and (C) Nlrc4^S533D/S533D mice. Sanger sequences traces are shown for mutant mice. Guide RNA sequence and protospacer-adjacent motif (PAM) for CRISPR/Cas9 targeting, as well as nonsilent base exchanges, are indicated. (D) Immunoblot (IB) of NLRC4 levels in bone marrow–derived macrophages. Representative of two independent experiments.