Skip to main content
. 2020 May 15;217(7):e20200004. doi: 10.1084/jem.20200004

Figure 4.

Figure 4.

Increased frequencies of antigen-specific T cells express CD57 and KLRG1 in CMV+ young adults. (A) Expression of AIMs OX40 and CD25 on CD4+ and CD8+ T cells in PBMCs that were unstimulated (unstim) or stimulated with CMV pp65, Ebola GP, or Staphylococcal enterotoxin B (SEB). (B and C) Frequency of OX40+CD25+ cells in CD4+ T cells (B) and CD8+ T cells (C) in CMV− (n = 8) and CMV+ (n = 8) individuals. Mann–Whitney analyses. (D) Frequency of CD57+KLRG1+ cells within GP-specific (OX40+CD25+) CD4+ and CD8+ T cells in CMV− (n = 8) and CMV+ (n = 8) UK adults at M+7. Mann–Whitney analyses between CMV− and CMV+ groups. (E–H) Relationship between frequency of CD57+KLRG1+ cells in CD4+ (E and G) or CD8+ (F and H) T cells and M+7 Ebola GP IFN-γ ELISPOT (E and F; CMV− n = 8, CMV+ n = 8) or IL2 produced by GP-stimulated (stim) M+7 PBMCs (G and H; measured using the LEGENDplex assay; CMV−, n = 8, and CMV+, n = 6; cytokine data for two individuals not available). Spearman’s rank analyses. SFC, spot-forming cell. Medians shown for all column graphs. Error bars indicate IQRs. *, P < 0.05; ***, P < 0.001.