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. 2020 May 6;217(7):e20191453. doi: 10.1084/jem.20191453

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© 2020 Tay et al.

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Figure 5. — HDAC3 negatively regulates a cytotoxic effector-associated transcriptional program in CD8 T cells. OT-I T cells were co-cultured with irradiated OVA peptide–pulsed BMDCs in vitro, and sorted to purity for molecular analysis. (A and B) Whole genome RNA-seq of Hdac3-KO and Hdac3-WT (A) or of RGFP966- and vehicle-treated (B) CD8 T cells 5 d after in vitro activation. Significance was determined using thresholds of −log₁₀(adjusted P value) >10 and |log₂(fold change)| >0.5. Data are from one experiment with three technical replicates per sample. TFs, transcription factors. (C) Venn diagram showing overlap between genes differentially expressed between Hdac3-KO and Hdac3-WT CD8 T cells, or RGFP966- and vehicle-treated CD8 T cells after 5 d of in vitro activation. Statistical analyses for RNA-seq data are described in the Materials and methods section. RNA-seq data are available through GEO accession no. GSE143644.