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. 2020 May 6;217(7):e20191453. doi: 10.1084/jem.20191453

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© 2020 Tay et al.

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Figure S2. — Characterization of thymopoiesis in E8I-Cre⁺; Hdac3^fl/flHdac3-KO mice. Related to Fig. 1. (A) Representative flow cytometry gating of live CD11b⁻ CD11c⁻ thymocyte populations in male 10-wk-old Hdac3-KO (E8I-Cre⁺; Hdac3^fl/fl) mice and Hdac3-WT (Cre⁻; Hdac3^fl/fl) littermates based on CD4 and CD8α expression. (B) Flow-cytometric measurement of thymocyte developmental stages, gated as in A. DD, CD4^lo CD8^lo double-dull; CD4, CD4⁺ CD8⁻ single-positive; CD8, CD4⁻ CD8⁺ single-positive thymocytes. (C and D) Flow-cytometric analysis (C) and quantification (D) of indicated thymocyte developmental stages in Hdac3-KO mice and Hdac3-WT littermates. (From left to right) Distribution of DN subsets, CD69^hi MHC I^lo DD cells undergoing negative selection, CD5^hi TCRβ⁺ DP cells undergoing positive selection, CD5⁺ mature CD4 SP, and CD5⁺ mature CD8 SP thymocytes. Data are representative of two independent experiments with three to five mice per genotype each. Means ± SD are indicated (B and D). P values were calculated by two-tailed Student’s t test (B and D). *, P < 0.05.