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. 2020 May 12;217(7):e20190742. doi: 10.1084/jem.20190742

Figure 4.

Figure 4.

STAT3 is required for Blimp-1 expression in T cells. Analysis of lung tissue isolated from control (STAT3f/f CD4Cre−) or STAT3CD4Cre (STAT3f/f CD4Cre+) animals i.n. immunized with HDM. (A) PAS staining (scale bar, 100 µm). (B) Percent of eosinophils in the BAL. (C) Percent of T cell subsets in lung (gated on live CD4+TCRβ+). (D) Percent of IL-10 producing effector T cells (FoxP3−) and T reg cells (FoxP3+). (E) Percent of Blimp-1+ effector T cells (gated on CD4+TCRβ+FoxP3−CD44+) in mLN and representative histogram. Data from B–E are pooled from two experiments with eight mice total per group, mean ± SD. (F and G) Percent of Th1 (IFN-γ+ T-bet+) and Th2 (IL-13+ GATA3+) cells (gated on CD4+TCRβ+FoxP3−; F), and (G) representative flow plots and percent of Blimp-1 YFP+ cells within Th1, Th2, and T reg (FoxP3+) cells in the lung after HDM. Data from F and G are pooled from two experiments with six to eight total mice per group, mean ± SD. Mann–Whitney t test (B–G). *, P < 0.05; ***, P < 0.001.