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. 2020 May 12;217(7):e20190742. doi: 10.1084/jem.20190742

Figure 5.

Figure 5.

IL-6 and IL-21 are not required for Blimp-1–mediated Th2 cell differentiation. (A and B) Percent of T cell subsets (A; gated on live CD4+ TCRβ+ FoxP3− [non-T reg] or FoxP3+ [T reg]) or (B) percent of Blimp-1+ cells (gated on Live, CD4+ TCRβ+ FoxP3− CD44+) isolated from lungs of control (IL-6Rαf/f CD4Cre−) or IL6RαCD4Cre (IL-6Rαf/f CD4Cre+) animals i.n. immunized with HDM. Data from A and B are pooled from two experiments with 10 mice per group, mean ± SD. (C–E) Percent of eosinophils in the BAL (C), (D) Th1 (IFN-γ+, T-bet+), and Th2 (IL-13+ GATA3+) cells (gated on live CD4+TCRβ+FoxP3−) and (E) percent of Blimp-1+ or Bcl6+ (gated on live CD4+TCRβ+FoxP3−) T cells in lung isolated from IL-21R–intact or IL-21R–deficient animals immunized with HDM. Data from C–E are pooled from two or three experiments with 9 or 10 total mice per group, mean ± SD. (F and G) Percent of Th1 (IFN-γ+ T-bet+) and Th2 (IL-13+ GATA3+) cells (gated on live CD4+TCRβ+FoxP3−; F) and (G) Blimp-1 YFP+ cells within Th1 and Th2 cells isolated from the lung from control (IgG) or anti–IL-21R–treated animals immunized with HDM. Data from F and G are pooled from two experiments with six or seven mice total per group, mean ± SD, Mann–Whitney t test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.