Figure S3.

Mice with endothelial Kras^G12D or Braf^V600E gain-of-function mutations exhibit normal proliferation of hepatic endothelial cells. (A and B) Ki67 (red) and Cdh5 (green) coimmunofluorescent staining with Hoechst nuclear counterstain (blue) on tamoxifen-treated Kras^{G12D F/+}; Cdh5^CreERT2 (A) and Braf^{V600E F/+}; Cdh5^CreERT2 (B) adult murine liver sections. White arrows show Ki67⁺ cells. n = 3. Scale bars: 100 µm (top row) and 10 µm (bottom row). Two independent experiments. FDR, false discovery rate. (C and D) Quantitation of proliferating Cdh5⁺ cells in tamoxifen-treated control, Kras^{G12D F/+}; Cdh5^CreERT2 (C), and Braf^{V600E F/+}; Cdh5^CreERT2 (D) embryonic murine livers. n = 3. Unpaired t test, P > 0.8 (C) and P > 0.4 (D). Two independent experiments. Data represent the mean ± SEM. (E and F) Ki67 (red) and Cdh5 (green) coimmunofluorescent staining with Hoechst nuclear counterstain (blue) on Kras^{G12D F/+}; Lyve1^Cre/+ (C) and Braf^{V600E F/+}; Lyve1^Cre/+ (D) embryonic murine liver sections. White arrows show Ki67⁺ cells. n = 3. E, embryonic day. Scale bars: 100 µm (top row) and 10 µm (bottom row). Two independent experiments. (G and H) Quantitation of proliferating Cdh5⁺ cells in control, Kras^{G12D F/+}; Lyve1^Cre/+ (G), and Braf^{V600E F/+}; Lyve1^Cre/+ (H) mutant murine livers. n = 3. Unpaired t test, P > 0.8. Two independent experiments. Data represent the mean ± SEM. N.S., not significant. BD, bile duct; LS, liver sinusoid; PV, portal vein.