Figure S4.

Mice with Kras^G12D or Braf^V600E gain-of-function mutations within macrophages exhibit normal normal sinusoidal and hepatic development. (A and B) Dissected Kras^{G12D F/+}; LysM^Cre (A) and Braf^{V600E F/+}; LysM^Cre (B) embryos show normal liver size (black arrows). H&E-stained Kras^{G12D F/+}; LysM^Cre (A) and Braf^{V600E F/+}; LysM^Cre (B) liver frontal sections show normal sinusoidal capillaries (black arrows). n = 3. E, embryonic day. Scale bars: 500 µm (top row), 1,000 µm (middle row), and 100 µm (bottom row). Two independent experiments. (C and D) Mice with Kras^G12D or Braf^V600E gain-of-function mutations within endothelial cells exhibit normal normal spleen morphology and sinusoids. Spleen dissected from adult tamoxifen-treated Kras^{G12D F/+}; Cdh5^CreERT2 (C) and Braf^{V600E F/+}; Cdh5^CreERT2 (D) mice. H&E-stained Kras^{G12D F/+}; Cdh5^CreERT2 (C) and Braf^{V600E F/+}; Cdh5^CreERT2 (D) spleen sagittal sections show normal morphology. Lyve1 (green) and Pecam1 (red) coimmunofluorescent staining with Hoechst nuclear counterstain (blue) on Kras^{G12D F/+}; Cdh5^CreERT2 (C) and Braf^{V600E F/+}; Cdh5^CreERT2 (D) spleen sagittal sections. n = 3. Scale bars: 500 µm (first and second rows), 100 µm (third row), and 50 µm (fourth row). Two independent experiments. White arrows show normal Lyve1⁺ vessels. A, artery; CrRL, cranial right lobe; LK, left kidney; LL, left lobe; LML, left medial lobe; LV, left ventricle; RK, right kidney; RML, right medial lobe; RV, right ventricle; S, stomach.