Figure S5.

Endothelial Kras^G12D or Braf^V600E gain-of-function mutations aberrantly enhance expression of adherens junctional proteins. (A) Analysis of differentially regulated transcripts show expression changes in E11.5 Braf^{V600E F/+}; Lyve1^Cre livers (n = 3). (B) KEGG pathway gene set enrichment analysis (GSEA) of differentially regulated, identified transcripts in E11.5 Braf^{V600E F/+}; Lyve1^Cre livers (n = 3). NES, normalized enriched score. (C and D) Flt4 (green) and Pecam1 (red) coimmunofluorescent staining with Hoechst nuclear counterstain (blue) on sections of murine Braf^{V600E F/+}; Lyve1^Cre embryonic liver (C) and murine Kras^{G12D F/+}; Lyve1^Cre liver (D). Red arrows show increased Flt4 expression. E, embryonic day. Scale bars: 100 µm (top row) and 10 µm (bottom row). n = 3. Two independent experiments. (E and F) Genotyping tables of embryos derived from Mapk1^F/+; Kras^{G12D F/+} mice (E) or Mapk1^F/+; Braf^{V600E F/+} (F) mice crossed with Mapk1^F/+; Lyve1^Cre/+ mice. χ² P values 0.262 (E) and 0.991 (F).